Pyruvate substituting lactate dialysis solution peritoneal resuscitation on the effects of hemorrhagic shock rabbit liver function / 实用医学杂志

Objective To observe the influence of direct peritoneal resuscitation on liver function in hemorrhagic shock rabbits with pyruvate peritoneal dialysis solution instead of lactate peritoneal dialysis solution. Methods 48 hemorrhagic shock rabbits were randomly divided into conventional intravenous resuscitation group (group A),intravenous resuscitation plus intraperitoneal injection of lactate peritoneal dialysis solution group (group B),intravenous resuscitation plus intraperitoneal injection of pyruvate peritoneal dialysis group(group C), intravenous pyruvate peritoneal dialysis resuscitation plus intraperitoneal injection of pyruvate peritoneal dialysis group(group D). The hemodynamic changes were observed and the AST and ALT were measured respectively before and 60 min after shock ,60 min and 180 min after resuscitation. The dry/wet weight ratio ,MDA and SOD of liver tissue were measured,and the liver morphological changes were observed. Results After the completion of shock resuscitation,MAP of all groups were almost restored to the basal level. 180 min after resuscitation,the MAP of animals in group A was lowered than that in group B,C and D(P<0.05). After shock,ALT and AST were signifi-cantly higher than those before shock ,but no significant difference was found between groups (P > 0.05). After resuscitation,ALT and AST continued to decline in all groups,but the decline range increased with the order of A, B,C,D group(P < 0.05). The water content and MDA in liver tissue decreased with the order of A,B,C,D group,while the SOD increased and the differences between groups were significant(P<0.05). And the degree of liver tissue morphological injury also relieved correspondingly. Conclusion Pyruvate substituting lactate peritoneal dialysis solution peritoneal resuscitation can be more effective in reducing liver damage of hemorrhagic shock rabbits.

Pyruvate substituting lactate dialysis solution peritoneal resuscitation on the effects of hemorrhagic shock rabbit liver function / 实用医学杂志

Objective To observe the influence of direct peritoneal resuscitation on liver function in hemorrhagic shock rabbits with pyruvate peritoneal dialysis solution instead of lactate peritoneal dialysis solution. Methods 48 hemorrhagic shock rabbits were randomly divided into conventional intravenous resuscitation group (group A),intravenous resuscitation plus intraperitoneal injection of lactate peritoneal dialysis solution group (group B),intravenous resuscitation plus intraperitoneal injection of pyruvate peritoneal dialysis group(group C), intravenous pyruvate peritoneal dialysis resuscitation plus intraperitoneal injection of pyruvate peritoneal dialysis group(group D). The hemodynamic changes were observed and the AST and ALT were measured respectively before and 60 min after shock ,60 min and 180 min after resuscitation. The dry/wet weight ratio ,MDA and SOD of liver tissue were measured,and the liver morphological changes were observed. Results After the completion of shock resuscitation,MAP of all groups were almost restored to the basal level. 180 min after resuscitation,the MAP of animals in group A was lowered than that in group B,C and D(P<0.05). After shock,ALT and AST were signifi-cantly higher than those before shock ,but no significant difference was found between groups (P > 0.05). After resuscitation,ALT and AST continued to decline in all groups,but the decline range increased with the order of A, B,C,D group(P < 0.05). The water content and MDA in liver tissue decreased with the order of A,B,C,D group,while the SOD increased and the differences between groups were significant(P<0.05). And the degree of liver tissue morphological injury also relieved correspondingly. Conclusion Pyruvate substituting lactate peritoneal dialysis solution peritoneal resuscitation can be more effective in reducing liver damage of hemorrhagic shock rabbits.

Effect of pyruvate replacing lactate dialysis solution peritoneal resuscitation on intestinal mucosal barrier of rabbit with hemorrhagic shock / 重庆医学

Objective To observe the effects of peritoneal resuscitation by pyruvate replacing lactate peritoneal dialysis solution on the intestinal mucosal barrier in rabbits with hemorrhagic shock.Methods The hemorrhagic shock model was prepared in 48 healthy male rabbits by adopting the improved Wiggers method.Then the rabbits were randomly divided into the conventional intravenous resuscitation group (group A),conventional intravenous resuscitation plus intraperitoneal injection of lactate peritoneal dialysis solution group (group B),conventional intravenous resuscitation plus intraperitoneal injection of pyruvate peritoneal dialysis solution group (group C) and intravenous pyruvate peritoneal dialysis solution resuscitation plus intraperitoneal injection of pyruvate peritoneal dialysis solution group (group D).The levels of diamine oxidase (DAO) and lipopolysaccharide (LPS) were measured before shock,at 60 min after shock,at 60,180 min after resuscitation.At the end of observation,the ileum tissues were taken from the 5 cm away from the ileocecal region for measuring the dry/wet ratio,malondialdehyde (MDA) level and superoxide dismutase (SOD) activity and their morphological changes was observed.Results There was no statistical difference in the plasma levels of DAO and LPS before shock among the four groups (P＞0.05);which at 50 min after shock were increased,but the comparison among the groups had no statistical difference (P＞0.05);the plasmas levels of DAO and LPS at 60,180 min after resuscitation in the group A,B,C and D were decreased in turn (P＜0.05).The MDA levels of ileum tissues after 180 min of resuscitation in the group A,B,C and D were decreased in turn (P＜0.05),but the SOD activties and tissue dry/wet mass ratios were increased in turn (P＜005),moreover the tissue injury degrees were also relieved in turn.Conclusion The peritoneal resuscitation by pyruvate replacing lactate peritoneal dialysis solution can more effectively protect the intestinal tissue cells and reduce intestinal mucosal damage in hemorrhagic shock rabbits.

Formulation of training programme and construction of curriculum system for foreign stu-dents in clinical medicine / 中华医学教育探索杂志

China Three Gorges University(CTGU) started undergraduate medical education for foreign students since 2004. Over the years,through continuous interaction with coordinators,CTGU grad-ually transited from passive teaching to active teaching. Firstly we determined basic principles,objectives and requirements of talent training programme. Secondly,we strengthen Chinese language teaching, reasonably adjusted professional curriculum and teaching content,added basic medical courses and rea-sonably arranged clinical practice based on the concrete situation in our country. in order to make the cur-riculum system consistent with the require-ments of talent training.

Management of Clinical Teaching Program by the Use of Excel Software / 中华医学教育探索杂志

Excel is electronic form software with strong function in Microsoft office. We utilize Excel software to manage the clinical teaching program. Clinical teaching program can be rapidly filled by way of duplicating, clipping, pasting, packing, arranging in order, and sieving etc. in the software, column numbers of teaching program can be added according to the need. After gathering teaching program of every research group of teaching into the total teaching program, we can conveniently arrange any column in an order and screens, so that the total teaching program was reset and optimized. The function of management of the total teaching program was so expanded that the quality and efficiency of clinical teaching management were raised.